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Expression and purification of SmTrx SmTrx was expressed, purified, and quantified using methods previously established ( Anaerobic techniques and transient-state conditions Following published protocols ( For each variant the reductive half-reaction was observed using both photomultiplier (PMT) detection at individual wavelengths specific to the observation and using charge coupled device (CCD) detection for all wavelengths between 300 and 800 nm

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