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Make sure that you thaw the frozen cells quickly but dilute them slowly using pre-warmed growth medium before plating

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J Assist Reprod Genet 22(910):361368 Parkhitko AA, Jouandin P, Mohr SE, Perrimon N (2019) Methionine metabolism and methyltransferases in the regulation of aging and lifespan extension across species

(PubMed) Evans M, Rumberger JA, Azumano I, Napolitano JJ, Citrolo D, Kamiya T

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