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The nanoparticles of silibinin exhibited antioxidant effects against intracellular oxidative stress by upregulating the Nrf2/ARE pathway, reducing ROS, modulating antioxidant enzyme responsiveness, and inhibiting downstream pathways of mitochondrial damage (Ding et al., 2022)

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The SQSTM1/p62 ELISA assay was first validated with established modulators of autophagic flux