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17 : 25962606 Miranda-Vizuete A, Rodriguez-Ariza A, Toribio F, Holmgren A, Lopez-Barea J and Pueyo C (1996) The levels of ribonucleotide reductase, thioredoxin, glutaredoxin 1, and GSH are balanced in Escherichia coli K12

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Even if the solution appears clear after thawing, freeze-thaw cycles denature proteins at the molecular level through mechanical shear and localized concentration gradients as ice forms

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GPX4 specific activity was carried out in a coupled assay, by monitoring the consumption of NADPH in the presence of glutathione reductase upon addition of phosphatidyl choline hydroperoxide ( Viability Assay Cells were seeded onto 96-well plates (5,000 cells per well) and allowed to attach overnight