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Tissue culture media supernatants from the final 24 hours of culture were collected and assayed by Amplex Red Glucose/Glucose Oxidase Assay Kit (Life Technologies, Gaithersburg, MD, USA), Lactate Assay kit (Sigma-Aldrich) and Rat Ultrasensitive Insulin ELISA (Mercodia, Uppsala, Sweden) to measure total glucose, lactate and insulin respectively

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This is especially so if you're also on other blood sugar-lowering medications or have health conditions that affect your body's sugar processing, such as kidney or pancreas disorders

Kidney sections from all groups demonstrated normal renal architecture with intact glomeruli and tubules

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