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Typically, cells were seeded in 100 L RPMI phenol red free media per well in duplicates on a black 96-well plate with clear bottom (Thermo Scientific)

For a 16:8 window with two meals, protein per meal needs to rise accordingly

The degree of MMSE score improvement correlated with the baseline Hcy levels and its reduction by folate supplementation

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CoQ10 is commonly used for egg mitochondrial support