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Under physiological conditions, renal tubular epithelial cells highly express the carnitine transporter OCTN2 (SLC22A5), which mediates active reabsorption of filtered carnitine with an efficiency exceeding 95%

Cell Culture The human colon cancer cell lines HCT116 and SW620, purchased from American Type Culture Collection (Manassas, VA, USA), were cultured in RPMI medium and DMEM/F12 (HyClone, Logan, UT, USA) respectively, supplemented with 10% foetal bovine serum (HyClone) and 1% penicillin and streptomycin

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Discard any unused portion.

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Cocamidopropyl Betaine is a fatty acid created by mixing similar compounds in coconut oil and dimethylaminopropylamine, a compound with two amino groups