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Antigen retrieval was performed using sodium citrate buffer (0.1 M, pH 6, with 0.05% Tween-20) in a microwave, then washed with de-ionized water and PBS on a shaker

2-adrenergic receptor antagonism attenuates CNV through inhibition of VEGF and IL-6 expression

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Cell 50 , 919930 (2013)