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Gene silencing by small interfering RNA Transient gene silencing was attained by transfection of small interfering RNA (siRNA) into cells using siPORT lipid transfection reagent according to the manufacturers instructions

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Lab results should be interpreted by a qualified healthcare provider

The 'growth factor' framing was the original description, before the copper-binding role of GHK was fully recognized

After washing two times in IC permeabilization buffer (cat#PB001C, BD Biosciences Pharmingen, CA, US), the cells were centrifuged at 1500 g for 4 min and the supernatant aspirated

In Canada, synthetic injectable peptides are generally regulated as prescription drugs under the Food and Drugs Act, and Health Canada has issued public advisories (April 2026 and earlier) cautioning that unauthorized injectable peptide products are illegal regardless of whether they are labelled 'for research use only.' Research-grade AOD-9604 supplied by Koipeptides is intended for in vitro laboratory study only and is not authorized for any human or veterinary application