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Avoid vigorous shaking during reconstitution, minimize moisture exposure of the lyophilized vial, protect the vial from light, and avoid repeated freeze-thaw cycles

Wakefulness time measurement To evaluate the impact of Pichia pastoris secreted recombinant peptides on insomnia, we analyzed the increase in wakefulness time among different groups of mice: Control, Model, GABA, DSIP-CBBBP, DSIP, and CBBBP