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Antibodies and reagents The anti--actin (cat#3700), anti--tubulin (cat#3873), anti-SLC7A11 (cat#12691), anti-ER (cat#13258), phospho-Tyr-100 (cat#9411), and phospho-ERK (Thr202/Tyr204) (cat#4370) were purchased from Cell Signaling Technology (Danvers, MA, USA)