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After standing for 10 min, the supernatant was removed and the remaining solid was dried in high vacuum to yield glutamate 1-semialdehyde (4-amino-5-oxopentanoic acid) as its hydrochloride salt

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A portion of the homogenate was tested for lipid peroxidation using spectrophotometry at 530 nm to estimate the amount of MDA produced.[] In order to measure the antioxidative enzyme activity, the remaining homogenate was centrifuged at 10,000 g at 4C for 10 min and analyzed colorimetrically

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The KEGG database is available for querying pathway information and signaling pathway retrieval, and so on

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