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Never use a pen or vial if the liquid appears cloudy, discolored, or contains particles

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For each z-slice, a relative signal was obtained by calculating the ratio intensity (mean gray value) for Ubx signal (488 nm) relative to the DAPI signal in T2-T3 segments (which only expressed ectopic Ubx protein) using the same region of interest (ROI)

Do not rub the skin after the injection

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The swelling in my neck visibly reduced, and over time the results just kept getting better