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Genetic screening of mtDNA mutations follows the criteria defined by DiMauro and Schon, 71 considering the presence of neutral polymorphisms, evolutionarily conserved and phenotypically distinct mutations, deletion mutations exhibiting heteroplasmy, and how the degree of heteroplasmy aligns with the severity of symptoms

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Rajala RV, Rajala A, Kooker C, Wang Y, Anderson RE

Determination of Michaelis-Menten constant for glutathione In these experiments, the concentration of monobromobimane was kept constant at 50 M, and concentration of glutathione was varied