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A properly lyophilized vial will dissolve readily when mixed with bacteriostatic water , producing a clear and colorless solution within minutes of gentle mixing

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Reduced inflammation and increased antioxidant enzymes

Samples were run on Tris-tricine or Tris-HCl SDS-PAGE gels and transferred to 0.45 m Immobilon-P Polyvinylidene Fluoride (PVDF) (Millipore) for Western blotting using ECL Prime substrate (GE Healthcare)