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A key component in this signaling cascade is phospholipase C (PLC), a crucial enzyme that, according to some scientific hypotheses, may facilitate the production of inositol triphosphate (IP3) and diacylglycerol (DAG)

Class B GPCRs typically bind their peptide ligands through a mechanism known as the two-domain model, in which ECD binds to the C-terminal helix of GLP-1, and the transmembrane domain (TMD) binds to the N-terminal region of GLP-1 (3, 4, 5)

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, 2-PA, NTA, AmP), and optimizing sample pretreatment ( e.g

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