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Peptides were eluted by addition of 300 l of buffer containing 50% HPLC grade acetonitrile, 50% HPLC grade H 2 O, 0.1% FA and the eluate was then evaporated to dryness in Speed vac

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RETA and SEMA administration The study design is shown in Supplementary Fig

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The reported half-life of Retatrutide is Approximately 6 days