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Dissolution in suitable solvent (e.g., water + acetonitrile) Reduction and alkylation (if disulfide bonds are present) Enzymatic digestion (optional for bottom-up sequencing approach) Key Insight: Clean and well-prepared samples significantly reduce noise and improve detection sensitivity

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[DOI] [PubMed] [Google Scholar] 40.Reedy J., Lerman J.L., Krebs-Smith S.M., Kirkpatrick S.I., Pannucci T., Wilson M.M., Subar A.F., Kahle L.L., Tooze J.A

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