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As with any therapeutic substance, it is advisable to consult a healthcare professional before starting BPC-157 to ensure safety and mitigate potential risks

After reaching 70% confluence, fibroblasts were cultured for 24 h in 1% FBS DMEM and then incubated with either TGF1 10ng/ml (Peprotech, Dollard des Ormeaux, Quebec, Canada), GHK 10 nM (Sigma, Markham, Ontario, Canada) or control media (1% FBS DMEM, penicillin, streptavidin) for a further 48 h

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Most sources recommend using reconstituted DSIP within 4 to 6 weeks, though some conservative guidelines suggest 2 to 4 weeks for optimal potency