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A structural analysis shows a C5/C6 spinal misalignment (subluxation)

Animal-serum-free blocking solution (Cell Signaling Technology, 15019L) diluted in H 2 0 was applied for 1 h, followed by incubation with the second primary antibody overnight

After discarding the medium, cells were fixed with 4% paraformaldehyde for 30 min, permeabilized with 0.5% Triton X-100 for 10 min, and then the reaction system was prepared as per the instructions

7 Blocking the export of intracellular GSH does not impair the ability of GSH to rescue in cystine-free conditions

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