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coli cells (100 mL) were collected (6000 rpm, 4 C, 10 min) and resuspended in 5 mL of lysis buffer containing 8 M urea, 4 % (w/v) CHAPS, and 1 % (w/v) DTT and sonicated on ice for 90 cycles (a working period of 3 s in a 6 s interval for each cycle) at a power output of 200 W by an ultrasonic disruptor (JY92-II, Scientz Biotechnology Co., Ningbo, China)

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This peptide is beneficial in repairing tissue and protects tissues from further damage

Saint Petersburg References Ota H, Igarashi S, Sato N, et al

Interestingly, this pattern reversed on day 14, with delayed proliferative fibroblastic cells in the NC group and few detected in the G/D-CuP group, indicating that the G/D-CuP treatment facilitates wound healing through a time-dependent mechanism