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Once in solution, the peptide-copper complex begins degrading immediately unless refrigerated

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Blots were incubated overnight at 4 C with HRP-conjugated anti-nitrotyrosine mouse mAb (1:2500 dilution in CanGetSignal solution 1,NKB-101, Toyobo, Osaka, Japan), followed by ECL-detection on a FUSION SOLO.7S.EDGE imaging system (Vilber Lourmat, Marne-la-Valle, France)

The endogenous decline of plasma GHK begins well before visible aging 1

Nakagaki K, Ebihara S, Usui S, Honda Y, Takahashi Y, Kato N