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Abstract Introduction: A recent study reported a favorable effect of vitamin B12 injections/oral folic acid support in myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) patients

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For protein purification, cell pellets of CDK2- and cyclin A2-expressing cells were combined and resuspended in purification buffer (50 mM HEPES pH 7.5, 180 mM NaCl, 5 % (v/v) glycerol, 2 mM MgCl 2 , 2 mM DTT) supplemented with protease inhibitors and DNase I