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When we compared the yields of GST-tagged fusion proteins as reported in the literature with the current study, we found few reports which suggested that GST is a poor solubility tag as compared to commonly used fusion tags as their protein yields were very low (Efna1 0.06 mg/L, CDK2 2.17 mg/L) [63]

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Louis, MO, USA) were used for determining their respective concentrations

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