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Equipment and LC-MS/MS conditions Chromatography: The chromatography analysis was performed using a sciex triple quad 6500+ LC-MS/MS equipped with a degasser model DGU-405, controller SCL-40 and CBM-40 LITE, pump LC-40D X3 and LC-40D XS, autosampler SIL-40C X3 and SIL-40C XS, and column oven CTO-40C and CTO-40S for the determination of semaglutide in human plasma samples

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It notes the clinical trials establishing semaglutide efficacy used consistent weekly schedules

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