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His and Arg act to help create the reactive environment, and Cys once again acts as the reaction center by using a thioester help hold a carboxyl group until the amine of a Lysine can perform a nucleophilic attack to transfer the protein and form the isopeptide bond

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Scott Brunner, CEO of the national Alliance for Pharmacy Compounding, called the CT officials' statements about the industry "distressing hyperbole at best and invited them to tour a compounding pharmacy

doi: 10.1016/j.molcel.2015.06.011 66 KoppulaPZhangYZhuangLGanB