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Once reconstituted with bacteriostatic water, 5-Amino-1MQ should be refrigerated at 28C and used within 28 days

The present study emulated a target trial to avoid immortal time and selection bias, chose a second-line glucose-lowering therapy as an active comparator to reduce confounding by indication, used an outcome definition based only on primary care records to minimize detection bias due to differential hospitalizations, and implemented a lag time to mitigate bias due to reverse causality

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