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Relative gene expression was determined by normalizing the expression of each target gene to -actin

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Commonly handled under cold-chain laboratory conditions, with frozen storage and light protection used to preserve sample integrity throughout the research period

Consequently, researchers can store it efficiently before reconstitution

Cold Spring Harb Perspect Med 2:a009332 Puerta E, Hervias I, Goi-Allo B et al (2010) Methylenedioxymethamphetamine inhibits mitochondrial complex I activity in mice: a possible mechanism underlying neurotoxicity

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