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U373 and HS683 cells (5 10) or U87 cells (3 10) were suspended in 300 L of serum-free MEM and seeded into the upper chamber, while 750 L of MEM containing 10% FBS was added to the lower chamber as a chemoattractant
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doi: 10.1210/endrev/bnac018 41
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Both protocols typically produce sleep improvements first, often within 1 to 2 weeks
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