Carbohydrates and fats blunt the GH response, so injections administered in a fasted state consistently produce stronger GH pulses
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In our study, we initially focused on mRNA analysis to understand gene expression dynamics, a choice that yielded valuable insights into transcriptional activities
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Materials and methods Cell culture The YUMMER1.7 (YR1.7) murine melanoma cell line (RRID: CVCL_A2AX) was established in our lab as previously described in 2017 [18]