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Engineered ECL1 linker residues are shown as dashed circles

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FCCP disrupts ATP production by transporting protons across the mitochondrial inner membrane, collapsing the proton gradient, and allowing the measurement of the maximum OCR, which reflects the electron transport chains capacity and substrate availability

(2) the ability of the TJ to selectively allow paracellular transport of certain ion species acting as ion channel and (3) the ability of the TJs to maintain cell polarity, by blocking the free diffusion of proteins and lipids between the apical and basolateral domains of the plasma membrane

The protein concentrations in cytosol and mitochondria samples were measured using the Bradford method