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PMID 10810093

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STAT3 signaling also triggers a negative feedback response to the exposure of insulin/IGF-1 and cytokine factors by inducing the expression of suppressor of cytokine signaling proteins (SOCS) (Fig

After harvesting by centrifugation, cells were lysed in a buffer containing Tris-HCl, EDTA, DTT, and lysozyme, then sonicated and centrifuged