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Vitamin B12 is naturally present almost exclusively in animal-derived foods, making vegans and strict vegetarians particularly vulnerable unless they consume fortified foods or supplements

5-(Biotinamido)pentylamine (BP) incorporation assay Purified GST tag and human TG2 were incubated with BP (Pierce), 5 mM 1,4-dithiothreitol (DTT) (Sigma-Aldrich), and CaCl 2 (Sigma-Aldrich)