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Prog Neurobiol 157:273291 Mancias JD, Wang X, Gygi SP, Harper JW, Kimmelman AC (2014) Quantitative proteomics identifies NCOA4 as the cargo receptor mediating ferritinophagy

Even identical prescriptions can show slight color variation between batches due to normal manufacturing tolerances

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NTS (240) were transferred to U-bottom 96 well assay plates containing 200 l of M199 medium supplemented with 5.5 mM D-glucose, penicillin-streptomycin and 5% heat inactivated fetal bovine serum and incubated at 37 C in a 5% CO 2 incubator overnight