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This multi-point binding mechanism creates a stable ternary complex (DNA-Mg-Pinealon) that can persist for sufficient duration to influence transcriptional machinery and chromatin structure, explaining the peptide's ability to produce long-lasting changes in gene expression patterns

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The results demonstrated a very strong accumulation of ROS inside the hypoxic cancer cells upon treatment with NP4 but a poor ROS accumulation upon preincubation with NAC and subsequent treatment with NP4 (flow cytometry plots: Fig