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To downregulate TfRs and limit the amount of surface binding or endocytosis in HL-60 cells, 100 M human holo-Tf (Sigma-Aldrich) in endotoxin-free PBS (EMD Millipore) was added to the medium for overnight incubation

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Patients should discuss their specific medication schedule with their surgeon and anesthesiologist well before the procedure date to establish a safe discontinuation timeline

Coverage determination, prior authorization and appeals workflows Plans should review GLP-1 related prior authorization and coverage determination workflows to confirm that Part D coverable uses continue to be handled through existing processes

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