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A comprehensive review on Raman spectroscopy applications

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Reconstitute the 1mg lyophilized vial in 10mM hydrochloric acid (HCl) at a concentration of 0.5-1.0 mg/mL to ensure complete solubilization, as IGF-1 LR3's isoelectric point near pH 8.4 makes it poorly soluble in neutral buffers and prone to aggregation at physiological pH

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