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Immunoprecipitation Hepatocytes were lysed in an immunoprecipitation (IP) buffer (150 mM NaCl), 50 mM Tris-HCl, 1% Nonidet P-40, pH 7.8, and a mammalian cell-specific protease inhibitor cocktail

Disaggregation of the minced tissue was achieved in 0.07% collagenase in the same HBSS solution supplemented with 4 mM CaCl2 and 1 mM deferoxamine