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In this and our previous study, we showed that inhibition of EPAC increases both cytoplasmic and mitochondrial ROS [7]

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Collection of blood and tissue samples At the end of the experimental period and following overnight fasting, rats were anesthetized using an intraperitoneal injection of ketamine (80 mg/kg body weight) and xylazine (10 mg/kg body weight)

The treatment itself involves a slow intravenous infusion that may be combined with other vitamins like vitamin C to boost the results

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As such, TBI research has focused on the treatment of secondary injury effects, which we will focus on in this review