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Slides were treated with HIER buffer (10 mM sodium citrate, 0.05% Tween-20, pH 6.0) for antigen retrieval in a pressure cooker for 10 min then sections were blocked in CAS-block histochemical reagent (Thermo Fisher Scientific, 008120)

Many approaches now incorporate both ABA and developmental or "relationship-based" models

In humans, a point mutation and deletion of the mitochondrial-encoding transfer RNA genes have also been known to associate with a specific form of diabetes known as mitochondrial diabetes [14]

doi: 10.1016/j.redox.2020.101600

It's an easy way to see if your body is getting what it needs from a hydration standpoint, and it's a key part of what medical professionals consider when they look at a basic urine sample

Additional information Competing interests The author(s) declare that they have no competing interests