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The PCR products were digested with Nco I and Bam HI and ligated into the pKM260 expression vector (EUROSCARF, Frankfurt, Germany), which resulted in the N-terminal addition of a hexahistidine tag and linker with the sequence MHHHHHHASENLYFQGAM

For context: a single jar of high-end topical copper peptide serum costs $60-150 and lasts a month

doi:10.1007/s10517-018-4219-2

Secondly, by initiating the program with many different initial conditions, we consistently arrived at the same periodic solution after a few months

In mammals, GST isoforms have cell specific distributions (for example, -GST in hepatocytes and -GST in the biliary tract of the human liver)