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At that point, tissue-level GHK-Cu has likely returned to baseline, and the cumulative signaling advantage of the loading phase is largely lost

Kang, W., Du, L., Liang, Q., Zhang, R., Lv, C., & Ge, S

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Consistently, we found that administration of CA, an endogenous FXR agonist, could not only significantly attenuate the T3-induced elevation of GLP-1 and insulin levels but also attenuate the glucose-lowering effect of T3 in MMI mice (Fig