The larvae developed into pupa and then matured into adult mosquitoes, which were maintained with water and 10% sucrose solution before their use in the experiments
Ding M, Bhupathiraju SN, Satija A, van Dam RM, Hu FB (2014) Long-term coffee consumption and risk of cardiovascular disease: a systematic review and a dose-response meta-analysis of prospective cohort studies
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Thurston L, et al