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Luminescence signal was measured using GloMax Explorer microplate reader (Promega Madison, WI, USA)

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It is not the same as AHK-Cu, a related tripeptide-copper complex (Ala-His-Lys with copper) that shares the histidine-anchored coordination chemistry but has different specific biological characterization

A total of 20 individual clones were sequenced from E3R3, E3R4 and E3R5