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Tian L, Huang ZJ, Na WD, Liu YY, Wang S, He Y, Cheng WJ, Huang TZ, Li Z, Li TX

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For method development and stability analysis, several crucial parameters must be optimized to ensure reliable separation: Method 0ptimization: Consistent retention and peak shape require shallow gradients, elevated column temperatures, and strong ion-pairing agents such as trifluoroacetic acid (TFA)

The cell line is ideal for screening candidate molecules for GLP-1 agonist activity and determining EC 50 dosing