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The transcriptomic results were verified by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis (Fig
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To control for intermolecular effects, a natural abundance sample of the MTSL-peptide was mixed in a 1:1 ratio with a 15 N-labeled Cys1253Ser/Cys1254Ser mutant S tail peptide, and peak intensities were measured for the oxidized and reduced states as above
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