decreased thick, organized collagen and elastin in the dermis
The survival rate, liver histomorphology, serum antioxidant enzyme activity, liver lipase activity and serum metabolomics, liver metabolomics were studied and analyzed by feeding the bullfrogs with abnormal lipid metabolism with glutathione for 20 days in the NC, FI and GSH groups
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After harvesting by centrifugation, cells were lysed in a buffer containing Tris-HCl, EDTA, DTT, and lysozyme, then sonicated and centrifuged
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