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The bound GLP-1 (right) stabilizes a water molecule network in the proximity of the peptide N-terminal residues H7 and A8 as well of Y1521.47, T3917.46, R1902.60, E3645.53

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Next, gonads were incubated for at least 1 hour in blocking solution (0.5% (w/v) bovine serum albumin diluted in PBStw) and incubated overnight at 4 with primary antibodies diluted in blocking solution as follows: mouse -V5 (1:1000, SV5-Pk1, Bio-Rad #MCA1360)

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