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Briefly, the gene encoding the OsDHAR sequence (residues 2203) was amplified by PCR using Ex Taq polymerase (TaKaRa Bio Inc., Shiga, Japan) with forward and reverse primers for a cDNA template (Table S1)

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Activation of PAMPs or DAMPs receptors promote the nuclear translocation of the NFkB dimer (p50/p65) that leads to the synthesis of the subunit (cRel) necessary for activating AANAT transcription