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The lysate was subsequently centrifuged (12000 g, 4 C, 5 min), denatured, analysed by SDSPAGE, and transferred to PVDF membranes (IPVH15150, Millipore)

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During the acclimatization period, the rats were fed a standard diet containing 23% crude protein, 7% crude fiber, 3% crude fat, 8% acid-insoluble ash, 12.5% calcium, 0.51% phosphorus, and 9% sodium

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Energy drinks can also be high in sodium